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airyscan joint deconvolution processing module  (Carl Zeiss)


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    Structured Review

    Carl Zeiss airyscan joint deconvolution processing module
    ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an <t>Airyscan</t> detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.
    Airyscan Joint Deconvolution Processing Module, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deconvolution+module/ZEN+Module+Deconvolution/pmc13043941-504-8-17
    Average 94 stars, based on 40 article reviews
    airyscan joint deconvolution processing module - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Plakoglobin transmits tension across VE-cadherin for vascular leak formation and leukocyte diapedesis"

    Article Title: Plakoglobin transmits tension across VE-cadherin for vascular leak formation and leukocyte diapedesis

    Journal: The EMBO Journal

    doi: 10.1038/s44318-026-00732-0

    ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an Airyscan detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.
    Figure Legend Snippet: ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an Airyscan detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.

    Techniques Used: Staining, Microscopy

    Related Articles

    Microscopy:

    Article Title: Dynamic Mitotic Localization of the Centrosomal Kinases CDK1, Plk, AurK, and Nek2 in Dictyostelium amoebae
    Article Snippet: .. Image acquisition and microscopy were performed at a Zeiss AxioObserver system equipped with a Zeiss Axiocam 506 mono, a PlanApo 1.4/100× lens, and ZEN 2012 Software (blue edition), including the iterative deconvolution module (Carl Zeiss Mikroskopie GmbH, Jena, Germany). ..

    Article Title: Modulators of HEC1 activity and methods therefor
    Article Snippet: DAPI (4′,6′-diamidino-2-phenylindole) staining was applied after secondary antibody incubation, and cells were mounted on cover slides with Prolong gold antifade reagent (Life Technologies, Carlsbad Calif. USA 92008). .. Images were captured with a Zeiss Axioplan 2 microscope equipped with a deconvolution module or with a Zeiss LSM-510 META laser scanning confocal microscope (Carl Zeiss Microscopy, Thomwood N.Y. 10594 USA). ..

    Article Title: Modulators of HEC1 activity and methods therefor
    Article Snippet: DAPI (4′,6′-diamidino-2-phenylindole) staining was applied after secondary antibody incubation, and cells were mounted on cover slides with Prolong gold antifade reagent (Life Technologies, Carlsbad Calif. USA 92008). .. Images were captured with a Zeiss Axioplan 2 microscope equipped with a deconvolution module or with a Zeiss LSM-510 META laser scanning confocal microscope (Carl Zeiss Microscopy, Thomwood N.Y. 10594 USA) FIG. 3 shows images of chromosomal misalignment in 110095-treated cancer cells and the effect of select Hec1 inhibitors on cell populations. ..

    Article Title: Partial Disassembly of the Nuclear Pore Complex Proteins during Semi-Closed Mitosis in Dictyostelium discoideum
    Article Snippet: .. Microscopy and image acquisition was performed at a Zeiss CellObserver HS system or an AxioObserver system equipped with a PlanApo 1.4/100× lens and Axiovision 4.8 software or ZEN Blue Software including the iterative deconvolution module (Carl Zeiss Mikroskopie GmbH, Jena, Germany). ..

    Software:

    Article Title: Dynamic Mitotic Localization of the Centrosomal Kinases CDK1, Plk, AurK, and Nek2 in Dictyostelium amoebae
    Article Snippet: .. Image acquisition and microscopy were performed at a Zeiss AxioObserver system equipped with a Zeiss Axiocam 506 mono, a PlanApo 1.4/100× lens, and ZEN 2012 Software (blue edition), including the iterative deconvolution module (Carl Zeiss Mikroskopie GmbH, Jena, Germany). ..

    Article Title: Defining the minimal structural requirements of DivIVA in filamentous Actinomycetota
    Article Snippet: To enable image deconvolution, point-spread functions (PSF) were determined using beads with excitation/emission wavelengths of 505/515 nm (green) and 633/660 nm (deep red) from the PS-Speck TM Microscope Point Source Kit (Invitrogen). .. The beads were imaged and PSFs calculated using the Deconvolution module in Zen software (Zeiss, ver 3.9). ..

    Article Title: Partial Disassembly of the Nuclear Pore Complex Proteins during Semi-Closed Mitosis in Dictyostelium discoideum
    Article Snippet: .. Microscopy and image acquisition was performed at a Zeiss CellObserver HS system or an AxioObserver system equipped with a PlanApo 1.4/100× lens and Axiovision 4.8 software or ZEN Blue Software including the iterative deconvolution module (Carl Zeiss Mikroskopie GmbH, Jena, Germany). ..

    Article Title: Essential and dispensable domains of DivIVA for walled growth in filamentous Actinomycetota
    Article Snippet: To enable image deconvolution, point-spread functions (PSF) were determined using beads with excitation/emission wavelengths of 505/515 nm (green) and 633/660 nm (deep red) from the PS-Speck Microscope Point Source Kit (Invitrogen). .. The beads were imaged and PSFs calculated using the Deconvolution module in Zen software (Zeiss, ver 3.9). ..

    Article Title: Essential and dispensable domains of DivIVA for walled growth in filamentous Actinomycetota.
    Article Snippet: To enable image deconvolution, point-spread functions (PSF) were determined using beads with excitation/emission wavelengths of 505/515 nm (green) and 633/660 nm (deep red) from the PS-Speck Microscope Point Source Kit (Invitrogen). .. The beads were imaged and PSFs calculated using the Deconvolution module in Zen software (Zeiss, ver 3.9). ..

    Article Title: Cancer Cells Can Exhibit a Sparing FLASH Effect at Low Doses Under Normoxic In Vitro- Conditions
    Article Snippet: Foci formation were assessed with a widefield fluorescence microscope, AxioOberver Z.1 (Zeiss, Oberkochen, Germany), equipped with ×63/1.40 Plan-Apochromat oil-immersion objective lens and Colibri 7 solid state LED light source (Zeiss), and an ORCA-Flash4.0 V3 Digital CMOS camera (Hamamatsu Photonics, Hamamatsu City, Japan). .. In each sample, at four different positions, fifteen Z-stack images were acquired, deconvoluted with a GPU-based deconvolution module and averaged using a Maximum Intensity Projection-algorithm (Black Zen Imaging Software, Zeiss). ..

    Imaging:

    Article Title: Cancer Cells Can Exhibit a Sparing FLASH Effect at Low Doses Under Normoxic In Vitro- Conditions
    Article Snippet: Foci formation were assessed with a widefield fluorescence microscope, AxioOberver Z.1 (Zeiss, Oberkochen, Germany), equipped with ×63/1.40 Plan-Apochromat oil-immersion objective lens and Colibri 7 solid state LED light source (Zeiss), and an ORCA-Flash4.0 V3 Digital CMOS camera (Hamamatsu Photonics, Hamamatsu City, Japan). .. In each sample, at four different positions, fifteen Z-stack images were acquired, deconvoluted with a GPU-based deconvolution module and averaged using a Maximum Intensity Projection-algorithm (Black Zen Imaging Software, Zeiss). ..



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    ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an <t>Airyscan</t> detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.
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    Image Search Results


    ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an Airyscan detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.

    Journal: The EMBO Journal

    Article Title: Plakoglobin transmits tension across VE-cadherin for vascular leak formation and leukocyte diapedesis

    doi: 10.1038/s44318-026-00732-0

    Figure Lengend Snippet: ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an Airyscan detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.

    Article Snippet: Zeiss LSM 980 Airyscan acquisitions were processed with Airyscan Joint Deconvolution processing module in ZEN blue software (Zeiss) to improve signal-to-noise and spatial resolution; LSM 880 images were used without further deconvolution.

    Techniques: Staining, Microscopy